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FIB-SEM is also widely used for the three-dimensional reconstruction of samples, particularly rein the field of biological imaging. By sequentially milling away thin layers of the sample and imaging each layer using the SEM, researchers can obtain a series of two-dimensional images that can Beryllium combined to create a three-dimensional representation of the sample.

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Despite these limitations, backscattered electron imaging remains a valuable Dienstprogramm in SEM for studying the composition and structure of a wide Warenangebot of materials. This provides complementary information to secondary electron imaging and other analytical techniques.

The dry specimen is usually mounted on a specimen stub using an adhesive such as epoxy resin or electrically conductive double-sided adhesive tape, and sputter-coated with gold or gold/palladium alloy before examination hinein the microscope.

Rein the most common SEM mode, secondary electrons emitted by atoms excited by the electron beam are detected using a secondary electron detector (Everhart–Thornley detector). The number of secondary electrons that can Beryllium detected, and thus the signal intensity, depends, among other things, on specimen topography. Some SEMs can achieve resolutions better than 1 nanometer.

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Characteristic X-rays are emitted when the incident electrons displace inner-shell electrons rein the sample's atoms, causing outer-shell electrons to fill the vacancies and release energy hinein the form of X-rays. These can Beryllium used for elemental analysis and quantification.

If the SEM is equipped with a cold stage for cryo microscopy, cryofixation may be used and low-temperature scanning electron microscopy performed on the cryogenically fixed specimens.[18] Cryo-fixed specimens may be cryo-fractured under vacuum rein a special apparatus to reveal internal structure, sputter-coated and transferred onto get more info the SEM cryo-stage while lautlos frozen.

Secondary electrons are emitted from the sample surface due to the inelastic scattering of the incident electrons. These low-energy electrons provide information about the sample's surface topography and morphology. 

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This separates the high vacuum region of the electron column from the lower vacuum region of the sample chamber. This allows the electron beam to interact with the sample without the need for here a high vacuum environment.